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avidin biotin complex kit  (Vector Laboratories)


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    Vector Laboratories avidin biotin complex kit
    Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+peroxidase/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13122667-89-14-17
    Average 99 stars, based on 12720 article reviews
    avidin biotin complex kit - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Anodal direct current stimulation of the auditory cortex at the onset of presbycusis delays cortical aging.
    Article Snippet: After washing three times with TBS-Tx for 15 min, the sections were incubated with an anti-rabbit biotinylated secondary antibody (biotinylated anti-rabbit IgG H + L, BA-1000; Vector, Burlingame, CA, USA) at a 1:200 dilution in TBS-Tx for 120 min, at room temperature. .. The sections were then washed with TBS-Tx and incubated for 180 min in avidin/biotin peroxidase (ABC complex, Vectastain Standard ABC kit PK-4000; Vector, Burlingame, CA, United States) and further washed with TBS-Tx, followed by Tris–HCl, pH 8.0. .. These sections were then incubated in 3.3 diaminobenzidine tetrahydrochloride (DAB; d-9015; Sigma-Aldrich, St. Louis, MO, United States) with 0.006% H2O2 and 0.4% nickel ammonium sulfate to visualize the peroxidase reaction.

    Article Title: Brainstem pathology in human narcolepsy: Neurodegeneration in the locus coeruleus in narcoleptic humans, but not in genetically narcoleptic mice or dogs
    Article Snippet: .. For secondary antibody, sections were incubated with biotinylated goat anti-rabbit IgG (1:500, PK-6101, Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue-bound peroxidase was visualized using a diaminobenzidine and nickel reaction (SK-4100, Vector Laboratories).

    Article Title: Brainstem pathology in human narcolepsy: Neurodegeneration in the locus coeruleus in narcoleptic humans, but not in genetically narcoleptic mice or dogs
    Article Snippet: Sections were then incubated in blocking solution (1% normal goat serum and 0.3% TritonX-100 in PBS) for 2 hours and subsequently incubated with dopamine beta-hydroxylase antibody (1:5000, rabbit monoclonal, Abcam, Cat. No. ab209487) for 72 hours at 4°C. .. Afterward, sections were incubated with biotinylated goat anti-rabbit IgG (1:500, PK-6101, Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue-bound peroxidase activity was visualized using a diaminobenzidine reaction (SK-4100, Vector Laboratories).

    Article Title: Anodal direct current stimulation of the auditory cortex at the onset of presbycusis delays cortical aging
    Article Snippet: After washing three times with TBS-Tx for 15 min, the sections were incubated with an anti-rabbit biotinylated secondary antibody (biotinylated anti-rabbit IgG H + L, BA-1000; Vector, Burlingame, CA, USA) at a 1:200 dilution in TBS-Tx for 120 min, at room temperature. .. The sections were then washed with TBS-Tx and incubated for 180 min in avidin/biotin peroxidase (ABC complex, Vectastain Standard ABC kit PK-4000; Vector, Burlingame, CA, United States) and further washed with TBS-Tx, followed by Tris–HCl, pH 8.0. .. These sections were then incubated in 3.3 diaminobenzidine tetrahydrochloride (DAB; d-9015; Sigma-Aldrich, St. Louis, MO, United States) with 0.006% H 2 O 2 and 0.4% nickel ammonium sulfate to visualize the peroxidase reaction.

    Article Title: Brainstem pathology in human narcolepsy: Neurodegeneration in the locus coeruleus in narcoleptic humans, but not in genetically narcoleptic mice or dogs
    Article Snippet: The sections were blocked in 1% normal goat serum in 0.01 M PBS for 2 hours and then incubated with tyrosine hydroxylase (1:10,000, rabbit polyclonal, Cat. No. AB 152, Millipore Sigma, MO) for 72 hours at 4°C. .. Sections were subsequently incubated with a secondary antibody (1:500, biotinylated goat anti-rabbit IgG, PK-6101, Vector Laboratories) followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours each at room temperature. .. Peroxidase activity was visualized with a diaminobenzidine reaction (SK-4100, Vector Laboratories).

    Article Title: Human narcolepsy is linked to degeneration of both locus coeruleus and hypocretin neurons.
    Article Snippet: The sections were blocked in 1% normal goat serum in 0.01 M PBS for 2 hours and then incubated with tyrosine hydroxylase (1:10,000, rabbit polyclonal, Cat. No. AB 152, Lot No. 4224636, Millipore Sigma, MO) for 72 hours at 4°C. .. Sections were subsequently incubated with a secondary antibody (1:500, biotinylated goat anti-rabbit IgG, PK-6101, Lot No. ZL0828 Vector Laboratories) followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours each at room temperature. .. Peroxidase activity was visualized with a diaminobenzidine reaction (SK-4100, Vector Laboratories).

    Article Title: Human narcolepsy is linked to degeneration of both locus coeruleus and hypocretin neurons.
    Article Snippet: .. For the secondary antibody, sections were incubated with biotinylated goat antirabbit IgG (1:500, PK-6101, Lot No. ZL0828 Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue- bound peroxidase was visualized using a diaminobenzidine and nickel reaction (SK-4100, Vector Laboratories).

    Article Title: Human narcolepsy is linked to degeneration of both locus coeruleus and hypocretin neurons.
    Article Snippet: Sections were then incubated in blocking solution (1% normal goat serum and 0.3% AR TI CL E IN P RE SS TritonX-100 in PBS) for 2 hours and subsequently incubated with dopamine beta-hydroxylase antibody (1:5000, rabbit monoclonal, Abcam, Cat. No. ab209487) for 72 hours at 4°C). .. Afterward, sections were incubated with biotinylated goat anti-rabbit IgG (1:500, PK-6101, Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue-bound peroxidase activity was visualized using a diaminobenzidine reaction (SK-4100, Vector Laboratories).

    Avidin-Biotin Assay:

    Article Title: Anodal direct current stimulation of the auditory cortex at the onset of presbycusis delays cortical aging.
    Article Snippet: After washing three times with TBS-Tx for 15 min, the sections were incubated with an anti-rabbit biotinylated secondary antibody (biotinylated anti-rabbit IgG H + L, BA-1000; Vector, Burlingame, CA, USA) at a 1:200 dilution in TBS-Tx for 120 min, at room temperature. .. The sections were then washed with TBS-Tx and incubated for 180 min in avidin/biotin peroxidase (ABC complex, Vectastain Standard ABC kit PK-4000; Vector, Burlingame, CA, United States) and further washed with TBS-Tx, followed by Tris–HCl, pH 8.0. .. These sections were then incubated in 3.3 diaminobenzidine tetrahydrochloride (DAB; d-9015; Sigma-Aldrich, St. Louis, MO, United States) with 0.006% H2O2 and 0.4% nickel ammonium sulfate to visualize the peroxidase reaction.

    Article Title: Brainstem pathology in human narcolepsy: Neurodegeneration in the locus coeruleus in narcoleptic humans, but not in genetically narcoleptic mice or dogs
    Article Snippet: .. For secondary antibody, sections were incubated with biotinylated goat anti-rabbit IgG (1:500, PK-6101, Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue-bound peroxidase was visualized using a diaminobenzidine and nickel reaction (SK-4100, Vector Laboratories).

    Article Title: Brainstem pathology in human narcolepsy: Neurodegeneration in the locus coeruleus in narcoleptic humans, but not in genetically narcoleptic mice or dogs
    Article Snippet: Sections were then incubated in blocking solution (1% normal goat serum and 0.3% TritonX-100 in PBS) for 2 hours and subsequently incubated with dopamine beta-hydroxylase antibody (1:5000, rabbit monoclonal, Abcam, Cat. No. ab209487) for 72 hours at 4°C. .. Afterward, sections were incubated with biotinylated goat anti-rabbit IgG (1:500, PK-6101, Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue-bound peroxidase activity was visualized using a diaminobenzidine reaction (SK-4100, Vector Laboratories).

    Article Title: Anodal direct current stimulation of the auditory cortex at the onset of presbycusis delays cortical aging
    Article Snippet: After washing three times with TBS-Tx for 15 min, the sections were incubated with an anti-rabbit biotinylated secondary antibody (biotinylated anti-rabbit IgG H + L, BA-1000; Vector, Burlingame, CA, USA) at a 1:200 dilution in TBS-Tx for 120 min, at room temperature. .. The sections were then washed with TBS-Tx and incubated for 180 min in avidin/biotin peroxidase (ABC complex, Vectastain Standard ABC kit PK-4000; Vector, Burlingame, CA, United States) and further washed with TBS-Tx, followed by Tris–HCl, pH 8.0. .. These sections were then incubated in 3.3 diaminobenzidine tetrahydrochloride (DAB; d-9015; Sigma-Aldrich, St. Louis, MO, United States) with 0.006% H 2 O 2 and 0.4% nickel ammonium sulfate to visualize the peroxidase reaction.

    Article Title: Brainstem pathology in human narcolepsy: Neurodegeneration in the locus coeruleus in narcoleptic humans, but not in genetically narcoleptic mice or dogs
    Article Snippet: The sections were blocked in 1% normal goat serum in 0.01 M PBS for 2 hours and then incubated with tyrosine hydroxylase (1:10,000, rabbit polyclonal, Cat. No. AB 152, Millipore Sigma, MO) for 72 hours at 4°C. .. Sections were subsequently incubated with a secondary antibody (1:500, biotinylated goat anti-rabbit IgG, PK-6101, Vector Laboratories) followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours each at room temperature. .. Peroxidase activity was visualized with a diaminobenzidine reaction (SK-4100, Vector Laboratories).

    Article Title: Human narcolepsy is linked to degeneration of both locus coeruleus and hypocretin neurons.
    Article Snippet: The sections were blocked in 1% normal goat serum in 0.01 M PBS for 2 hours and then incubated with tyrosine hydroxylase (1:10,000, rabbit polyclonal, Cat. No. AB 152, Lot No. 4224636, Millipore Sigma, MO) for 72 hours at 4°C. .. Sections were subsequently incubated with a secondary antibody (1:500, biotinylated goat anti-rabbit IgG, PK-6101, Lot No. ZL0828 Vector Laboratories) followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours each at room temperature. .. Peroxidase activity was visualized with a diaminobenzidine reaction (SK-4100, Vector Laboratories).

    Article Title: Human narcolepsy is linked to degeneration of both locus coeruleus and hypocretin neurons.
    Article Snippet: .. For the secondary antibody, sections were incubated with biotinylated goat antirabbit IgG (1:500, PK-6101, Lot No. ZL0828 Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue- bound peroxidase was visualized using a diaminobenzidine and nickel reaction (SK-4100, Vector Laboratories).

    Article Title: Human narcolepsy is linked to degeneration of both locus coeruleus and hypocretin neurons.
    Article Snippet: Sections were then incubated in blocking solution (1% normal goat serum and 0.3% AR TI CL E IN P RE SS TritonX-100 in PBS) for 2 hours and subsequently incubated with dopamine beta-hydroxylase antibody (1:5000, rabbit monoclonal, Abcam, Cat. No. ab209487) for 72 hours at 4°C). .. Afterward, sections were incubated with biotinylated goat anti-rabbit IgG (1:500, PK-6101, Vector Laboratories) for 2 hours at room temperature, followed by avidin-biotin-peroxidase (1:300, PK-6101, Vector Laboratories) for 2 hours. .. The tissue-bound peroxidase activity was visualized using a diaminobenzidine reaction (SK-4100, Vector Laboratories).



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